Own topic Essay Example | Topics and Well Written Essays - 1250 words

Own topic - Essay ExampleHow the temperature of the solution influences the enzyme activity. It is expected that the enzyme will be undo at high temperatures and will work slowly at low temperatures because the enzyme is found in a plant that lives approximately at 10C-30C.The influence of the pH on enzyme activity. Because the plant tissue that the enzyme is found in is not extremely acidic or basic, it is expected that extremes of pH will have a blackball notion on enzyme activity.Previously prepared enzyme extract at chromas of 0.5, 1.0 and 2.0 ml was added to tubes containing 1.0 ml guaicol dye (or 0 ml for control), 2.0 ml H2O2 (or 0 ml for control), and soften (pH 5) at volumes of 3-5 ml to produce a total volume of 8.0 ml. Spectrophotometer readings at 500 nm were performed at 20 second intervals immediately after coalesce the tubes for two minutes and the results were recorded.Mixtures of 4 ml buffer (pH 5) (or 0 ml for control), 2.0 ml H2O2 (or 0 ml for control), 1.0 ml of extract (or 0 ml for control) and 1.0 ml guaicol dye (or 0 ml for control) were combined at a total volume of 8 ml, incubated at 4C, 22C, 32C and 48C and 100CAll the solutions were pre-incubated at the appropriate temperatures for 15 minutes to allow them to equilibrate before mixing. Spectrophotometer readings at 500 nm were performed at 20 second intervals immediately after mixing the tubes for two minutes and the results were recorded.To project the effects of pH mixtures of 4 ml buffer at pH 3, 5, 7 or 9 (or 5 or 0 ml for control), 2.0 ml H2O2 (or 0 ml for control), 1.0 ml of extract (or 0 ml for control) and 1.0 ml guaicol dye (or 0 ml for control) were combined at a total volume of 8 ml. Spectrophotometer readings at 500 nm were performed at 20 second intervals immediately after mixing the tubes for two minutes and the results were recordedTo see how inhibitors effect enzyme activity mixtures of 4 ml buffer (pH 5) (or 0 ml